Journal
NUCLEIC ACIDS RESEARCH
Volume 50, Issue 12, Pages 6854-6869Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkac515
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Funding
- National Institute of Environmental Health Sciences [R35ES028343]
- National Institutes of General Medical Sciences [1RM1GM130450]
- National Institutes of Child Health and Human Development, National Institutes of Health
- National Institute of Neurological Disorders and Stroke, National Institutes of Health [R21NS114911]
- National Science Foundation [CHE-1664801]
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Homologs of mutagenic DNA polymerase V are encoded by pathogens and mobile elements. These polymerases can be transferred horizontally into different cells but require activation by host factors.
Homologs of the mutagenic Escherichia coli DNA polymerase V (pol V) are encoded by numerous pathogens and mobile elements. We have used Rum pol (RumA ' B-2), from the integrative conjugative element (ICE), R391, as a model mobile element-encoded polymerase (MEPol). The highly mutagenic Rum pol is transferred horizontally into a variety of recipient cells, including many pathogens. Moving between species, it is unclear if Rum pol can function on its own or requires activation by host factors. Here, we show that Rum pol biochemical activity requires the formation of a physical mutasomal complex, Rum Mut, containing RumA ' B-2-RecA-ATP, with RecA being donated by each recipient bacteria. For R391, Rum Mut specific activities in vitro and mutagenesis rates in vivo depend on the phylogenetic distance of host-cell RecA from E. coli RecA. Rum pol is a highly conserved and effective mobile catalyst of rapid evolution, with the potential to generate a broad mutational landscape that could serve to ensure bacterial adaptation in antibiotic-rich environments leading to the establishment of antibiotic resistance.
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