Journal
NEW ZEALAND JOURNAL OF AGRICULTURAL RESEARCH
Volume 66, Issue 4, Pages 366-373Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/00288233.2022.2080721
Keywords
Sheep; KRTAP27-1; variation; wool traits; keratin-associated protein; mean staple length
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This study identified the ovine KRTAP27-1 gene using PCR-SSCP analysis and found four sequence variants, including two non-synonymous SNPs in the coding region. The study also discovered that sheep with genotypes AB and BB had longer fibers and higher wool weight compared to sheep with genotype AA.
The keratin-associated proteins (KAPs) are structural components of wool and hair fibres, with over 90 genes that encode these proteins having been identified in mammalian species. A KAP gene called KRTAP27-1 was recently identified in goats, but the gene has not been reported in the closely related ruminant species sheep. In this study, we used a polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis to find ovine KRTAP27-1 and investigate nucleotide sequence variation in the gene. Our analysis revealed four sequence variants (named A to D) in 399 New Zealand Merino x Southdown-cross sheep. Within these sequence variants there were five single nucleotide polymorphisms (SNPs) in the coding region, including two non-synonymous SNPs. Six genotypes (AA, AB, AC, BB, BC and BD) of KRTAP27-1 were detected, and for the three genotypes with a frequency of over 5.0%, sheep of genotypes AB and BB produced wool of higher mean staple length (MSL) and higher greasy fleece weight (GFW) than sheep of genotype AA. These results suggest that variation in ovine KRTAP27-1 may be affecting wool growth and thus production.
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