Journal
JOURNAL OF DAIRY SCIENCE
Volume 99, Issue 2, Pages 1002-1010Publisher
ELSEVIER SCIENCE INC
DOI: 10.3168/jds.2015-9993
Keywords
acid tolerance response; Lactobacillus plantarum; physiological and transcriptional level; cross-protection
Funding
- National Natural Science Foundation of China [NSF31170091, 31360377, 31260363]
- Ganpo Talent Engineering 555 Project
- Academic and Technical Leaders Training Program for Major Subjects of Jiangxi Province (P. R. China)
- Research Project of Jiangxi Provincial Education Department (P. R. China) [GJJ13098]
- Postdoctoral Science Foundation Funded Project of China [M570567]
Ask authors/readers for more resources
Acid tolerance responses (ATR) in Lactobacillus plantarum ZDY2013 were investigated at physiological and molecular levels. A comparison of composition of cell membrane fatty acids (CMFA) between acid-challenged and unchallenged cells showed that acid adaptation evoked a significantly higher percentage of saturated fatty acids and cyclopropane fatty acids in acid-challenged than in unchallenged cells. In addition, reverse transcription-quantitative PCR analysis in acid-adapted cells at different pH values (ranging from 3.0 to 4.0) indicated that several genes were differently regulated, including those related to proton pumps, amino acid metabolism, sugar metabolism, and class I and class III stress response pathways. Expression of genes involved in fatty acid synthesis and production of alkali was significantly upregulated. Upon exposure to pH 4.5 for 2 h, a higher survival rate (higher viable cell count) of Lactobacillus plantarum ZDY2013 was achieved following an additional challenge to 40 mM hydrogen peroxide for 60 mm, but no difference in survival rate of cells was found with further challenge to heat, ethanol, or salt. Therefore, we concluded that the physiological and metabolic changes of acid-treated cells of Lactobacillus plantarum ZDY2013 help the cells resist damage caused by acid, and further initiated global response signals to bring the whole cell into a state of defense to other stress factors, especially hydrogen peroxide.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available