4.8 Editorial Material

Towards a full picture of the total transcriptome

Related references

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Article Biotechnology & Applied Microbiology

High-throughput total RNA sequencing in single cells using VASA-seq

Fredrik Salmen et al.

Summary: VASA-seq is a method that can detect the total transcriptome in single cells by fragmenting and tailing all RNA molecules after cell lysis. It is compatible with different experimental formats and can be used to analyze cell type markers, in vivo cell cycle, and alternative splicing, among others.

NATURE BIOTECHNOLOGY (2022)