Journal
NATURE GENETICS
Volume 54, Issue 7, Pages 963-+Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41588-022-01100-4
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Funding
- National Cancer Centre Research Fund
- National Medical Research Association Clinician Scientist Award [MOH-CSAINV17nov-0003]
- Agency for Science, Technology and Research, Singapore [H18/01/a0/020]
- National Research Council Clinician Scientist-Individual Research Grant [CIRG21jun-0038]
- IAF-ICP grant [I1901E0041]
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This study classifies tumor cells into two epithelial subtypes in colorectal cancer patients through single-cell transcriptomic analysis, and reveals differences in activation of pathways and metastasis based on epithelial subtype, microsatellite stability, and fibrosis. The findings improve tumor classification and provide a better understanding of colorectal cancer. The two identified subtypes are validated in a larger cohort through bulk transcriptomic analysis, demonstrating their stability and feasibility. Furthermore, this study defines the intrinsic epithelial axis of colorectal cancer and proposes a refined classification method based on intrinsic epithelial subtype, microsatellite instability status, and fibrosis.
A single-cell transcriptomic analysis of 63 patients with colorectal cancer classifies tumor cells into two epithelial subtypes. An improved tumor classification based on epithelial subtype, microsatellite stability and fibrosis reveals differences in pathway activation and metastasis. The consensus molecular subtype (CMS) classification of colorectal cancer is based on bulk transcriptomics. The underlying epithelial cell diversity remains unclear. We analyzed 373,058 single-cell transcriptomes from 63 patients, focusing on 49,155 epithelial cells. We identified a pervasive genetic and transcriptomic dichotomy of malignant cells, based on distinct gene expression, DNA copy number and gene regulatory network. We recapitulated these subtypes in bulk transcriptomes from 3,614 patients. The two intrinsic subtypes, iCMS2 and iCMS3, refine CMS. iCMS3 comprises microsatellite unstable (MSI-H) cancers and one-third of microsatellite-stable (MSS) tumors. iCMS3 MSS cancers are transcriptomically more similar to MSI-H cancers than to other MSS cancers. CMS4 cancers had either iCMS2 or iCMS3 epithelium; the latter had the worst prognosis. We defined the intrinsic epithelial axis of colorectal cancer and propose a refined 'IMF' classification with five subtypes, combining intrinsic epithelial subtype (I), microsatellite instability status (M) and fibrosis (F).
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