Journal
NATURE BIOTECHNOLOGY
Volume 40, Issue 11, Pages 1680-+Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41587-022-01347-6
Keywords
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Categories
Funding
- ISMMS seed fund
- Dean's office grant
- National Cancer Institute (NCI) Cancer Center Support grant [P30 CA196521]
- NCI [T32CA078207]
- Charles H. Revson Foundation
- Instituto de Salud Carlos III [COV20-00668, PI16CIII/00012]
- Instituto de Salud Carlos III, Spanish Ministry of Science and Innovation [COV20/00181]
- European Development Regional Fund 'A way to achieve Europe'
- Instituto de Salud Carlos III, Spain [COV20/00170]
- Government of Cantabria, Spain [2020UIC22-PUB-0019]
- Fondo Social Europeo e Iniciativa de Empleo Juvenil YEI [PEJ2018-004557-A]
- European Union [101037867]
- [U24CA224319]
- [U01DK124165]
- [NCI K00CA212474]
- [REDInREN 016/009/009 ISCIII]
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This study reports the development of two quantitative PCR assays for detecting SARS-CoV-2-specific T cell activation. These assays are rapid, internally normalized, and can quantify the magnitude and duration of cellular immune responses, helping to determine revaccination strategies in vulnerable populations.
The T cell response to SARS-CoV-2 is detected by a PCR assay on whole blood. Fast, high-throughput methods for measuring the level and duration of protective immune responses to SARS-CoV-2 are needed to anticipate the risk of breakthrough infections. Here we report the development of two quantitative PCR assays for SARS-CoV-2-specific T cell activation. The assays are rapid, internally normalized and probe-based: qTACT requires RNA extraction and dqTACT avoids sample preparation steps. Both assays rely on the quantification of CXCL10 messenger RNA, a chemokine whose expression is strongly correlated with activation of antigen-specific T cells. On restimulation of whole-blood cells with SARS-CoV-2 viral antigens, viral-specific T cells secrete IFN-gamma, which stimulates monocytes to produce CXCL10. CXCL10 mRNA can thus serve as a proxy to quantify cellular immunity. Our assays may allow large-scale monitoring of the magnitude and duration of functional T cell immunity to SARS-CoV-2, thus helping to prioritize revaccination strategies in vulnerable populations.
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