Journal
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
Volume 43, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.nano.2022.102553
Keywords
DNA nano-networks; Multivalent aptamers; Drug delivery; Targeted cancer therapy
Funding
- National Natural Science Foundation of China (NSFC) [22174020]
- Key Project of Natural Science Foundation of Fujian Province [2019J02005]
- Research Foundation of Education Bureau of Hunan Province [19B384]
- Open Project of State Key Laboratory of Chemo/biosensing and Chemometrics [2016010]
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Structural DNA nanotechnology has great potential in applications such as bioimaging, drug delivery, and cancer therapy. This study demonstrates an intelligent aptamer-incorporated DNA nanonetwork for cancer cell imaging and targeted drug delivery. The nanonetwork can transform into nanosheet intermediates and specifically enter target cells through cancer cell-surface receptors, showing increased binding affinity and maintaining structural integrity.
The structural DNA nanotechnology holds great potential application in bioimaging, drug delivery and cancer therapy. Herein, an intelligent aptamer-incorporated DNA nanonetwork (Apt-Nnes) is demonstrated for cancer cell imaging and targeted drug delivery, which essentially is a micron-scale pattern with the thickness of double-stranded monolayer. Cancer cell-surface receptors can make it perform magical transformation into small size of nanosheet intermediates and specifically enter target cells. The binding affinity of Apt-Nnes is increased by 3-fold due to multivalent binding effect of aptamers and it can maintain the structural integrity in fetal bovine serum (FBS) for 8 h. More interestingly, target cancer cells can cause the structural disassembly, and each resulting unit transports 4963 doxorubicin (Dox) into target cells, causing the specific cellular cytotoxicity. The cell surface receptor-mediated disassembly of large size of DNA nanostructures into small size of fractions provides a valuable insight into developing intelligent DNA nanostructure suitable for biomedical applications.(c) 2022 Elsevier Inc. All rights reserved.
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