Journal
MOLECULAR BIOLOGY REPORTS
Volume 49, Issue 12, Pages 12109-12119Publisher
SPRINGER
DOI: 10.1007/s11033-022-07704-7
Keywords
CRISPR; Cas; Genome editing; Potato; Virus resistance; Plant
Categories
Funding
- ICAR-CPRI, Shimla
- CABin Scheme (ICAR-IASRI, New Delhi)
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Plant viruses cause significant yield loss in potatoes, and CRISPR-Cas genome editing technology has emerged as a powerful tool for developing virus-resistant varieties. This article highlights the applications of CRISPR-Cas in potatoes, including targeting viral genome and host factor genes for enhancing virus resistance, and the latest advancements in related research.
Plant viruses are the major pathogens that cause heavy yield loss in potato. The important viruses are potato virus X, potato virus Y and potato leaf roll virus around the world. Besides these three viruses, a novel tomato leaf curl New Delhi virus is serious in India. Conventional cum molecular breeding and transgenics approaches have been applied to develop virus resistant potato genotypes. But progress is slow in developing resistant varieties due to lack of host genes and long breeding process, and biosafety concern with transgenics. Hence, CRISPR-Cas mediated genome editing has emerged as a powerful technology to address these issues. CRISPR-Cas technology has been deployed in potato for several important traits. We highlight here CRISPR-Cas approaches of virus resistance through targeting viral genome (DNA or RNA), host factor gene and multiplexing of target genes simultaneously. Further, advancement in CRISPR-Cas research is presented in the area of DNA-free genome editing, virus-induced genome editing, and base editing. CRISPR-Cas delivery, transformation methods, and challenges in tetraploid potato and possible methods are also discussed.
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