Journal
MICROCHIMICA ACTA
Volume 189, Issue 9, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-022-05419-x
Keywords
Loop-mediated isothermal amplification; Point-of-need detection; E. coli O157:H7; Salmonella spp; S. aureus
Categories
Funding
- National Key Research and Development Program of China [2022YFE0201800]
- Shenzhen-Hong Kong-Macao Science and Technology Plan Project [SGDX2020110309260000]
- Guangdong Natural Science Foundation [2021A1515220020]
- Shenzhen Science and Technology Innovation Commission [JCYJ20210324140004013, GJHZ20210705142200001]
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This study developed a cost-effective, portable, and easy-to-use on-site genetic diagnostics sensing system for pathogen screening. The system consists of a paper-based biochip and a smartphone-based portable device, which together enable simultaneous detection of multiple pathogens and have demonstrated practicality in foodborne pathogen detection.
The development of cost-effective, portable, and ease-of-use sensing system for on-site genetic diagnostics is highly desirable for pathogen screening and infectious disease diagnosis. This study develops (1) a paper-based biochip which is able to integrate the loop-mediated isothermal amplification (LAMP) protocols for simultaneous detection of Escherichia coli O157:H7, Salmonella spp., and Staphylococcus aureus, and (2) a stand-alone smartphone-based portable device which can control exactly 65 degrees C for isothermal amplification as well as collect and analyze the thus generated fluorescence signals. The reported sensing system has been successfully demonstrated for foodborne pathogen detection with a limit of detection of 2.8 x 10(-5) ng mu L-1. Spiked milk samples with concentration as low as 10 CFU mL(-1) were successfully determined within 4 h, demonstrating the practicality of the reported sensing system in the fields. The reported sensing system featuring simplicity and reliability is ideally suited for genetic diagnostics in low resource settings.
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