Early detection of multiple sclerosis (MS) as a neurodegenerative disease using electrochemical nano-aptasensor

Multiple sclerosis (MS) is a progressive inflammatory disease (neurodegenerative), and its diagnosis is very important. MicroRNAs (miR) are small, non-coding RNAs that are abnormally expressed in many diseases and have been identified as predictive biomarkers for the early detection of diseases. One of the best diagnostic biomarkers of MS is miR-155. In this report, a sensitive, low-cost, non-label electrochemical nano biosensor was used for miR-155 detection. The biosensor comprises a nanocomposite of single-walled carbon nanotubes (SWCNT) and polypyrrole (PPY) on the graphite sheet (GS) substrate which was modified with an aptamer as miR-155 capture probe. All electrochemical measurements were performed in the presence of Fe(CN)(6)(3-/4-)as a redox probe. The biosensor has a dynamic range of 10 aM to 1 mu M with a detection limit of 10 aM. Also, the fabricated biosensor was successfully used in human serum. According to the ease of preparation, almost low cost, good selectivity, repeatability, and reproducibility the proposed biosensor can be a promising candidate for early detection of MS.

Automated summary

In this study, a sensitive, low-cost, label-free electrochemical nano biosensor was used to detect the diagnostic biomarker miR-155 for multiple sclerosis (MS). The biosensor demonstrated a wide dynamic range and low detection limit, and was successfully applied in human serum.