Journal
MICROCHEMICAL JOURNAL
Volume 179, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.microc.2022.107551
Keywords
Fluorescent microspheres lateral flow assay; MicroRNAs; Point-of-care testing; Rolling circle amplification; Simultaneous detection; Smartphone
Categories
Funding
- Space Medical Experi-ment Project of China Manned Space Program [HYZHXM04003]
- Science and Technology Innovation Program of Beijing Institute of Technology [1870011162001]
Ask authors/readers for more resources
This study developed a portable and multiple miRNAs detection method, combining rolling circle amplification with fluorescent microspheres based lateral flow assay. A smartphone-based strip reader with a WeChat mini program was used to analyze the fluorescence intensity of test lines and control lines on strips for point-of-care testing.
More and more attention has been paid to microRNAs (miRNAs) detection, as they have been recognized as a kind of important disease-related biomarkers. However, due to the costly and bulky instruments as well as highly skilled and complex operations, the existing detection methods have some limitations in miRNA detection, especially in low-resource areas. The present study developed a method integrated rolling circle amplification (RCA) with fluorescent microspheres (FM) based lateral flow assay (LFA) (RCA-FM-LFA) for portable and multiple miRNAs detection. Particularly, a smartphone-based strip reader with WeChat mini program was designed to analyze the fluorescence intensity of test lines (T lines) and control lines (C lines) on strips for meeting the requirements of point-of-care testing (POCT). The proposed method was sensitive, specific, accurate, relatively fast and portable. Under optimal conditions, the concentrations of miRNA 21 and miRNA let-7a as low as 230.60 pM and 27.89 pM could be simultaneously detected with only 2 mu L of sample needed within 3 h, and no interference can be caused by non-target miRNAs or serum matrix. Satisfactory recoveries ranging from 85.00% to 106.83% for miRNA 21 and from 94.40% to 107.33% for miRNA let-7a were obtained in spiked artificial serum. With further development, the proposed method herein can be chosen as a potential analytical tool for miRNAs detection, which might play an important role in early screening, diagnosis, and prognosis of human serious diseases.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available