Journal
JOURNAL OF CRYSTAL GROWTH
Volume 469, Issue -, Pages 42-47Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jcrysgro.2016.09.029
Keywords
Crystallisation; Protein; 3D nanotemplates; Protein mixture; Surface Porosity; Purification
Funding
- National Natural Science Foundation of China [21474059]
- Engineering and Physical Sciences Research Council [EP/N015916/1] Funding Source: researchfish
- EPSRC [EP/N015916/1] Funding Source: UKRI
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This study reports an experimental validation of the surface preferential nucleation of proteins on the basis of a relationship between nucleant pore diameter and protein hydrodynamic diameter. The validated correlation was employed for the selection of nucleant pore diameter to crystallise a target protein from binary, equivolume protein mixture. We report proof-of-concept preliminary experimental evidence for the rational approach for crystallisation of a target protein from a binary protein mixture on the surface of 3D nanotemplates with controlled surface porosity and narrow pore-size distribution selected on the basis of a relationship between the nucleant pore diameter and protein hydrodynamic diameter. The outcome of this study opens up an exciting opportunity for exploring protein crystallisation as a potential route for protein purification and bio-separation in both technical and pharmaceutical applications. (C) 2016 Elsevier B.V. All rights reserved.
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