Antioxidant Activity of Fucoidan Modified with Gallic Acid Using the Redox Method

Antioxidant compounds decrease the amount of intracellular reactive oxygen species (ROS) and, consequently, reduce the deleterious effects of ROS in osteoblasts. Here, we modified a 21 kDa fucoidan (FucA) with gallic acid (GA) using the redox method, to potentiate its antioxidant/protective capacity on pre-osteoblast-like cells (MC3T3) against oxidative stress. The 20 kDa FucA-GA contains 37 +/- 3.0 mg GA per gram of FucA. FucA-GA was the most efficient antioxidant agent in terms of total antioxidant capacity (2.5 times), reducing power (five times), copper chelation (three times), and superoxide radical scavenging (2 times). Exposure of MC3T3 cells to H2O2 increased ROS levels and activated caspase-3 along with caspase-9. In addition, the cell viability decreased approximately 80%. FucA-GA also provided the most effective protection against oxidative damage caused by H2O2 . Treatment with FucA-GA (1.0 mg/mL) increased cell viability (similar to 80%) and decreased intracellular ROS (100%) and caspase activation (similar to 80%). In addition, Fuc-GA (0.1 mg/mL) abolished H2O2-induced oxidative stress in zebra fish embryos. Overall, FucA-GA protected MC3T3 cells from oxidative stress and could represent a possible adjuvant for the treatment of bone fragility by counteracting oxidative phenomena.

Automated summary

Antioxidant compounds reduce intracellular reactive oxygen species (ROS) and protect osteoblasts from ROS-induced damage. This study demonstrates that modifying a 21 kDa fucoidan with gallic acid can enhance its antioxidant capacity and protect pre-osteoblast-like cells against oxidative stress. The modified FucA-GA showed significantly higher antioxidant activity compared to other compounds, effectively reducing ROS levels and caspase activation. It also demonstrated promising protective effects against oxidative damage in zebra fish embryos. Overall, FucA-GA could have potential therapeutic applications for bone fragility by counteracting oxidative stress.