Journal
JOURNAL OF TRANSLATIONAL MEDICINE
Volume 20, Issue 1, Pages -Publisher
BMC
DOI: 10.1186/s12967-022-03568-4
Keywords
Primary hyperparathyroidism; Transcriptome sequencing; DNA methylation sequencing; POMC
Categories
Funding
- National Natural Science Foundation of China [81860147]
Ask authors/readers for more resources
In this study, the transcriptome and DNA methylation profiles of parathyroid adenoma were analyzed, and several candidate genes that may be involved in the pathogenesis of PHPT were identified. Among them, hypermethylation of the POMC promoter may contribute to its low expression, which may be involved in the development of PHPT.
Primary hyperparathyroidism (PHPT) is mainly caused by parathyroid adenoma, which produces excess parathyroid hormones. Its pathogenic mechanisms have not yet been fully understood. To investigate the mechanism in the pathogenesis of PHPT, the transcriptome and genome-wide DNA methylation profiles of parathyroid adenoma were analyzed. The candidate genes that may be involved in the PHPT were verified via qRT-PCR, immunohistochemistry, western blot, and methylation-specific PCR. A total of 1650 differentially expressed genes and 2373 differentially methylated regions were identified. After the integration of its transcriptome and DNA methylation data, IL6, SYP, GNA01, and pro-opiomelanocortin (POMC) were the candidate genes that demonstrated a similar pattern between their mRNA expression and DNA methylation status. Of the 4 candidate genes, POMC, a pro-peptide which is processed to a range of bioactive peptide products like ACTH, was further confirmed to be expressed at low levels at both the mRNA and protein levels, which may be due to POMC promoter hypermethylation. Hypermethylation of the POMC promoter may contribute to its low expression, which may be involved in the pathogenesis of PHPT.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available