Journal
JOURNAL OF CROHNS & COLITIS
Volume 10, Issue 6, Pages 735-746Publisher
OXFORD UNIV PRESS
DOI: 10.1093/ecco-jcc/jjw024
Keywords
Metagenomics; metatranscriptomics; metaproteomics; 16S; meta-omics; microbiota
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Meta-omics [metagenomics, metatranscriptomics, and metaproteomics] are rapidly expanding our knowledge of the gut microbiota in health and disease. These technologies are increasingly used in inflammatory bowel disease [IBD] research. Yet, meta-omics data analysis, interpretation, and among-study comparison remain challenging. In this review we discuss the role these techniques are playing in IBD research, highlighting their strengths and limitations. We give guidelines on proper sample collection and preparation methods, and on performing the analyses and interpreting the results, reporting available user-friendly tools and pipelines.
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