Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 144, Issue 24, Pages 10700-10704Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jacs.2c03188
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Funding
- European Research Council [ERC AdG 669628]
- Deutsche Forschungsgemeinschaft [SE819/231]
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This study introduces a new reaction design involving the template-controlled cleavage of PNA-spermine conjugates, allowing for catalytic activity and high efficiency. It has potential applications in DNA-encoded drug discovery and nucleic acid diagnostics.
Nucleic-acid-templated chemical reactions are currently explored for applications in DNA-encoded drug discovery, nucleic acid diagnostics, and theranostics. Of particular interest are reactions enabling the template to gain catalytic activity, so that enzymatic amplification of low copy targets would no longer be necessary. Herein, we introduce a new reaction design relying on the template-controlled cleavage of PNA-spermine conjugates. With turnover frequencies in the range of 3-10 min-1 and a kcat/KM = 1.3 x 106 M-1 s-1, the loss of affinity upon reaction provides a catalytic efficiency equal to most enzymatic conversions and superior to nucleic-acid-templated reactions reported to date.
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