Journal
JOURNAL OF SEPARATION SCIENCE
Volume 45, Issue 15, Pages 3054-3062Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.202200351
Keywords
enrichment; magnetic separation; O-phospho-L-tyrosine; phosphoprotein
Categories
Funding
- National Natural Science Foundation of China [81772941]
- Key Program of the National Natural Science Foundation [81930066]
Ask authors/readers for more resources
Phosphorylation is a crucial post-translational modification that influences the function of proteins. This study developed a simple method to prepare titanium (IV)-immobilized magnetic nanoparticles for efficient enrichment of phosphoproteins. The results demonstrated excellent enrichment performance and high selectivity, making this method a promising approach for phosphoprotein research.
Phosphorylation is one of the most important protein post-translational modifications, which possesses dramatic regulatory effects on the function of proteins. In consideration of the low abundance and low stoichiometry of phosphorylation and non-specific signal suppression, efficient capture of the phosphoproteins from complex biological samples is critical to meet the need for protein profiling. In this work, a facile preparation of titanium (IV)-immobilized O-phospho-Ltyrosine modified magnetic nanoparticles was developed for the enrichment of intact phosphoproteins. The prepared magnetic nanoparticles were characterized by various instruments and had a spherical shape with an average diameter of 300 nm. The adsorption isotherms were investigated and the maximum capacity for beta-casein was calculated to be 961.5 mg/g. Standard protein mixtures and biological samples (non-fat milk and human serum) were selected to test the enrichment performance. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis demonstrated the excellent enrichment performance with high selectivity. With the superparamagnetic property, titanium (IV)-immobilized O-phospho-L-tyrosine modified magnetic nanoparticles were convenient for the practical application and clinical promotion, thus having a promising prospect in the field of phosphoprotein research.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available