4.7 Article

The truncated AaActin1 promoter is a candidate tool for metabolic engineering of artemisinin biosynthesis in Artemisia annua L.

Journal

JOURNAL OF PLANT PHYSIOLOGY
Volume 274, Issue -, Pages -

Publisher

ELSEVIER GMBH
DOI: 10.1016/j.jplph.2022.153712

Keywords

Artemisia annua; AaActin1 promoter; Promoter activity; Artemisinin biosynthesis; 5 ' intron deletion

Categories

Funding

  1. National Key R&D Program of China [2018YFA0900600]
  2. Bill & Melinda Gates Foundation [OPP1199872]
  3. National Natural Science Foundation of China [31770327]
  4. SJTU Global Strategic Partnership Fund
  5. SJTU Transmed Awards Research [20190104]
  6. Bill and Melinda Gates Foundation [OPP1199872] Funding Source: Bill and Melinda Gates Foundation

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A new promoter, tpACT, was discovered in this study, showing higher activity than the commonly used CaMV35S promoter in artemisinin-producing plants. Application of tpACT in genetic transformation of Artemisia annua can increase the production of artemisinin.
Malaria is a devastating parasitic disease with high levels of morbidity and mortality worldwide. Artemisinin, the active substance against malaria, is a sesquiterpenoid produced by Artemisia annua. To improve artemisinin content in the native A. annua plants, considerable efforts have been attempted, with genetic transformation serving as an effective strategy. Although, the most frequently-used cauliflower mosaic virus (CaMV) 35S (CaMV35S) promoter has proved to be efficient in A. annua transgenic studies, it appears to show weak activity in peltate glandular secretory trichomes (GSTs) of A. annua plants. Here, we characterized the 1727 bp fragment upstream from the translation start codon (ATG) of AaActin1, however, found it was inactive in tobacco. After removal of the 5' intron, the truncated AaActin1 promoter (tpACT) showed 69% and 50% activity of CaMV35S promoter in transiently transformed tobacco and stably transformed A. annua, respectively. beta-glucuronidase (GUS) staining analysis showed that the tpACT promoter was capable of directing the constant expression of a foreign gene in peltate GSTs of transgenic A. annua, representing higher activity than CaMV35S promoter. Collectively, our study provided a novel promoter available for metabolic engineering of artemisinin biosynthesis in A. annua.

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