QTL mapping for cold tolerance and higher overwintering survival rate in winter rapeseed (Brassica napus)

The cold tolerance of winter rapeseed (Brassica napus) cultivars is critically important for winter survival and yield formation in northern China. Few studies have examined the genetic mechanism underlying the overwintering survival of B. napus. Here, an F-2 population including 174 lines and an F-2:3 population including 174 lines were generated to identify the quantitative trait loci (QTLs) related to the cold tolerance of B. napus. A genetic linkage map including 1,017 markers merged into 268 bins covering 793.53 cM was constructed. A total of 16 QTLs for two cold-tolerance indicators related to overwintering success were detected among the two populations. These QTLs were responsible for explaining 0.97%-12.74% of the phenotypic variation. Two major QTLs, qOWRTA07 and qOWRLA07, explaining more than 10% of the phenotypic variation were identified in overlapping regions, and we suspected that these two QTLs might represent the same QTL mapped between the two bins, c07b004 and c07b005, corresponding to the physical interval from 21.4 M to 23.4 M on chromosome A07. One gene, BnaA07G0198300ZS, contained the candidate region for overwintering rate (OWR). RT-qPCR analysis showed that the expression of this gene significantly differed between the two parents (NST57 and CY12), and its expression was higher in NST57 than in CY12. This gene may be involved in the cold-response during overwintering period of B. napus. These results are important for the molecular breeding to improve the cold tolerance and overwintering success of winter oilseed rape.

Automated summary

This study identified the genetic mechanism underlying the cold tolerance of winter rapeseed (Brassica napus) and discovered QTLs and candidate genes related to winter survival. These results are important for improving the cold tolerance and overwintering success of winter oilseed rape.