A novel nano material for anti-cerebral ischaemia: preparation and application of borneol angelica polysaccharide liposomes

Objective: To investigate the preparation of novel nanoliposomes (Borneol Angelica Polysaccharide Liposomes, BAPL) for anti-cerebral ischaemia and verify its curative effects and mechanism. Methods: By applying a uniform experiment design to investigate the fitting combination of BAPL. Encapsulation Efficiency Evaluation of BAPL Preparation; Particle Size and Surface Potential Evaluation of BAPL Biological activity; Cerebral ischaemia models of rats Evaluation of BAPL curative effects and mechanism. Results: (1) The fitting combination of lecithin, Cholesterol, AP mass and the borneol mass was 60 mg, 60 mg, 45 mg and 5 mg. the highest encapsulation efficiency was 80.4%, the particle size was 179.1 nm, and the surface zeta potential was -17.2 mV. It conforms to the nano-material standards. (2) The results of animal experiments show that: In the BAPL group, the infarct volume of TTC staining was significantly decreased, and the expression levels of NF-kappa Bp65, TLR-4, IL-8, IL-6, IL-1 beta in brain tissue were significantly decreased, while the expression levels of ZO-1, ZO-2, IL-10 were significantly increased after cerebral ischaemia-reperfusion. Conclusion: BAPL is a novel nano and effective material for anti-cerebral ischaemia.

Automated summary

Objective of this study was to prepare novel nanoliposomes (Borneol Angelica Polysaccharide Liposomes, BAPL) for anti-cerebral ischaemia and investigate its curative effects and mechanism. The optimal combination of ingredients for BAPL was determined, resulting in high encapsulation efficiency and suitable particle size and surface charge. Animal experiments confirmed that BAPL significantly reduced infarct volume and modulated the expression levels of key proteins in brain tissue. Thus, BAPL is a promising nano material for the treatment of cerebral ischaemia.