Multi-omics analysis reveals the influence of tetracycline on the growth of ryegrass root

Environmental factors, including antibiotics such as tetracycline, can alter biological processes in plants. To ascertain how cell/tissue response to tetracycline, a multi-omic analysis was implemented to explore the molecular mechanism of tetracycline influencing the growth of ryegrass root. Tetracycline induced extensive changes in the root metabolome in plants, particularly impacting metabolites of flavonoid metabolic pathways, which were supported through consistent differences between transcriptome and proteome. Cross-comparison between mRNA and protein contents considered the authentication of congruence with related metabolites and revealed changes of several biological processes under tetracycline stress. Overall, we present an undemanding multi-omic strategy to survey the significant influence on the root under tetracycline stress.

Automated summary

This study employed a multi-omic analysis to investigate the molecular mechanism of tetracycline's influence on ryegrass root growth. The results showed that tetracycline induced extensive changes in root metabolism in plants, particularly affecting metabolites in the flavonoid metabolic pathways. Cross-comparison between transcriptome, proteome, and metabolome revealed changes in several biological processes under tetracycline stress.