Insights into the human gut virome by sampling a population from the Indian subcontinent

Gut virome plays an important role in human physiology but remains poorly understood. This study reports an investigation of the human gut DNA-virome of a previously unexplored ethnic population through metagenomics of faecal samples collected from individuals residing in Northern India. Analysis shows that, similar to the populations investigated earlier, majority of the identified virome belongs to bacteriophages and a smaller fraction (< 20 %) consists of viruses that infect animals, archaea, protists, multiple domains or plants. However, crAss- like phages, in this population, are dominated by the genera VI, VII and VIII. Interestingly, it also reveals the presence of a virus family, Sphaerolipoviridae, which has not been detected in the human gut earlier. Viral families, Siphoviridae, Myoviridae, Podoviridae, Microviridae, Herelleviridae and Phycodnaviridae are detected in all of the analysed individuals, which supports the existence of a core virome. Lysogeny-associated genes were found in less than 10 % of the assembled genomes and a negative correlation was observed in the richness of bacterial and free -viral species, suggesting that the dominant lifestyle of gut phage is not lysogenic. This is in contrast to some of the earlier studies. Further, several hundred high-quality viral genomes were recovered. Detailed characterization of these genomes would be useful for understanding the biology of these viruses and their significance in human physiology.

Automated summary

This study investigated the gut DNA-virome of an unexplored ethnic population in Northern India and found that most of the identified virome belongs to bacteriophages, with a smaller fraction consisting of viruses that infect animals, archaea, protists, multiple domains or plants. The study also discovered a previously undetected virus family in the human gut. Additionally, the research revealed that the dominant lifestyle of gut phages in this population is lysogenic, which contradicts some earlier studies.