Journal
JOURNAL OF CONTROLLED RELEASE
Volume 235, Issue -, Pages 134-146Publisher
ELSEVIER
DOI: 10.1016/j.jconrel.2016.05.055
Keywords
Green designed lipoprotein-like nanoparticles; Bioinspired disassembly-reassembly strategy; Multi-targeting system; Enhanced anticancer efficacy; Safety profile
Funding
- National Natural Science Foundation of China [81273469, 81501582, 81573379]
- Operating Funds for Basic Sciences Research in Central Universities [2015PY011]
- Development Funds for Priority Academic Programs in Jiangsu Higher Education Institutions, Innovation Projects in Novel Drugs by University Students [J1030830]
- Fostering Plan of University Scientific and Technology Innovation Team of Jiangsu Qing Lan Project
Ask authors/readers for more resources
In this study, a simple and green approach 'bioinspired disassembly-reassembly strategy' was employed to reconstitute lipoprotein nanoparticles (RLNs) using whole-components of endogenous ones (contained dehydrated human lipids and native apolipoproteins). These RLNs were engineered to mimic the configuration and properties of natural lipoproteins for efficient drug delivery. In testing therapeutic targeting tomicrotubules, paclitaxel (PTX) was reassembled into RLNs to achieve improved targeted anti-carcinoma treatment and minimize adverse effects, demonstrating ultimately more applicable than HDL-like particles which are based on exogenous lipid sources. We have characterized that apolipoprotein-decoration of PTX-loaded RLNs (RLNs-PTX) led to favoring uniformly dispersed distribution, increasing PTX-encapsulation with a sustained-release pattern, while enhancing biostability during blood circulation. The innate biological RLNs induced efficient intracellular trafficking of cargos in situ via multi-targeting mechanisms, including scavenger receptor class B type I (SR-BI)-mediated direct transmembrane delivery, as well as other lipoprotein-receptors associated endocytic pathways. The resulting anticancer treatment from RLNs-PTX was demonstrated a half-maximal inhibitory concentration of 0.20 mu g/mL, cell apoptosis of 18.04% 24 h post-incubation mainly arresting G2/M cell cycle in vitro, and tumor weight inhibition of 70.51% in vivo. Collectively, green-step assembly-based RLNs provided an efficient strategy for mediating tumor-targeted accumulation of PTX and enhanced anticancer efficacy. (C) 2016 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available