Journal
JOURNAL OF CONTROLLED RELEASE
Volume 222, Issue -, Pages 159-168Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jconrel.2015.12.017
Keywords
Cell penetrating peptide; Topical; Nucleic acid delivery; Skin penetration; Dermatology; Transfection
Funding
- Council of Scientific and Industrial Research (CSIR), New Delhi, India (Network Project TOUCH) [BSC0302]
Ask authors/readers for more resources
Topical delivery to skin is an essential step in non-invasive application of nucleic acid therapeutics for cutaneous disorders. The barrier posed by different layers of the skin - stratum corneum on top followed by the viable epidermis below - makes it extremely challenging for large hydrophilic molecules like nucleic acids to efficiently enter the uncompromised skin. We report an amphipathic peptide Mgpe9 (CRRLRHLRHHYRRRWHRFRC) that can penetrate the uncompromised skin, enter skin cells and deliver plasmid DNA efficiently as nanocomplexes in vitro and in vivo without any additional physical or chemical interventions prevalent currently. We observe efficient gene expression up to the highly proliferating basal layer of the skin without observable adverse reactions or toxic effects after delivery of reporter plasmids. The entry mechanism of nanocomplexes possibly involves reversible modulation of junction proteins accompanied by transient changes in skin structure. This peptide holds potential to be used as an efficient transporter of therapeutic nucleic acids to the skin. (C) 2015 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available