Smartphone-compatible, CRISPR-based platforms for sensitive detection of acute hepatopancreatic necrosis disease in shrimp

Article Fisheries

The lymphoid organ (LO) is an additional, prime target for decapod iridescent virus 1 (DIV1) in Penaeus vannamei

Piyachat Sanguanrut et al.

Summary: The study confirmed histopathological changes caused by DIV1 virus infection in whiteleg shrimp through detailed analysis, providing a quicker method for histological diagnosis of DIV1 infection.

AQUACULTURE (2022)

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Article Fisheries

CRISPR-based platform for rapid, sensitive and field-deployable detection of scale drop disease virus in Asian sea bass (Lates calcarifer)

Thanwarat Sukonta et al.

Summary: A novel detection platform for SDDV based on CRISPR-Cas12a and recombinase polymerase amplification technology has been developed, showing high sensitivity and specificity for rapid and accurate diagnosis of SDDV. This user-friendly method can be completed within an hour at room temperature, potentially serving as a valuable tool for combating large-scale epidemics in fish farming communities.

JOURNAL OF FISH DISEASES (2022)

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Article Veterinary Sciences

CRISPR/Cas12a Based Rapid Molecular Detection of Acute Hepatopancreatic Necrosis Disease in Shrimp

Chenglong Li et al.

Summary: A new molecular assay combining RPA and CRISPR/Cas12a technology was developed to detect the virulence genes causing AHPND in shrimp. The assay showed rapid and specific detection, with the addition of a lateral flow strip readout for visualization. Field tests demonstrated high predictive agreement.

FRONTIERS IN VETERINARY SCIENCE (2022)

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Article Chemistry, Multidisciplinary

Figure of Merit for CRISPR-Based Nucleic Acid-Sensing Systems:Improvement Strategies and Performance Comparison

Reza Nouri et al.

Summary: This study developed a quantitative figure of merit (FOM) for CRISPR sensing to compare different methods and explore performance improvement strategies. By evaluating the FOM performances of existing studies, the effectiveness of strategies to improve FOM was found to be consistent with the model prediction.

ACS SENSORS (2022)

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Article Fisheries

Infectious myonecrosis virus (IMNV) and Decapod iridescent virus 1 (DIV1) detected in captured, wild Penaeus monodon

Jiraporn Srisala et al.

Summary: IMNV and DIV1 are lethal viruses for shrimp, first discovered in 2004 and 2016 respectively, with 99-100% sequence identity in positive tests on captured P. monodon. The study highlights the potential risk of introducing these viruses to shrimp hatcheries and farms through apparently healthy captured P. monodon and recommends surveillance and precautions be taken.

AQUACULTURE (2021)

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Article Chemistry, Multidisciplinary

Machine Learning-Driven and Smartphone-Based Fluorescence Detection for CRISPR Diagnostic of SARS-CoV-2

Aubin Samacoits et al.

Summary: The paper introduces a cost-effective smartphone-based device combined with machine learning software for CRISPR diagnosis of SARS-CoV-2. The device has a detection limit of 6.25 RNA copies/μL in laboratory samples, with a test accuracy of 95% and sensitivity of 97% on nasopharyngeal swab samples with transmissible viral loads.

ACS OMEGA (2021)

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Article Fisheries

Establishment of a visualized isothermal nucleic acid amplification method for on-site diagnosis of acute hepatopancreatic necrosis disease in shrimp farm

Xiaohan Yang et al.

Summary: AHPND is a significant deadly disease in the shrimp farming industry, and the RPA-LFS diagnostic method offers a quick response time, easy procedure, and low resource demand, with significant value in controlling the spread of this dangerous disease in farmed shrimp.

JOURNAL OF FISH DISEASES (2021)

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Article Biochemical Research Methods

Development of a Recombinase Polymerase Amplification (RPA) assay for acute hepatopancreatic necrosis disease (AHPND) detection in Pacific white shrimp (Penaeus vannamei)

Hung Nam Mai et al.

Summary: AHPND, caused by Vibrio spp. carrying pirA and pirB genes, is a major bacterial disease in shrimp industry, diagnosed mainly by PCR but limited for point-of-care diagnostics. An isothermal RPA assay was developed for AHPND detection, showing high specificity and sensitivity with a detection limit of 5 copies of pirAB genes, making it a reliable alternative method for AHPND detection outside a standard laboratory setup.

MOLECULAR AND CELLULAR PROBES (2021)

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Review Food Science & Technology

Acute Hepatopancreatic Necrosis Disease (AHPND): Virulence, Pathogenesis and Mitigation Strategies in Shrimp Aquaculture

Vikash Kumar et al.

Summary: Shrimp, a high-protein animal food commodity, is a fast-growing food producing sector globally. Disease outbreaks, particularly acute hepatopancreatic necrosis disease (AHPND) caused by Vibrio spp., have led to significant production losses and socio-economic and environmental challenges in the shrimp aquaculture industry. Current conventional approaches to mitigate AHPND, such as antibiotics and disinfectants, have shown limited success and have been linked to antibiotic resistance in bacterial pathogens.

TOXINS (2021)

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Article Biochemistry & Molecular Biology