Journal
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
Volume 919, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jelechem.2022.116544
Keywords
Impedimetric biosensor; Glucose oxidase; Ferrocene; Nafion; Porous graphene; Central composite design
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Funding
- Payame Noor University
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In this study, a novel impedimetric glucose biosensor was fabricated using a biocomposite material and successfully applied for the analysis of real human blood serum samples. The biosensor showed a wide linear range, high sensitivity, and low detection limit. The fabrication process of the biosensor was simple and straightforward.
A novel Impedimetric glucose biosensor was fabricated based on a biocomposite of porous graphene, Nafion, Ferrocene, and glucose oxidase drop-casted on a glassy carbon electrode. The biocomposite components ratios optimization was studied by the central composite design method. Biosensor structural characterizations and fabrication process was performed by various spectroscopy, microscopy, and electrochemical techniques, including Raman spectroscopy, electrochemical impedance spectroscopy, scattered energy X-ray spectroscopy, atomic force microscopy, scanning electron microscopy, and cyclic voltammetry. The fabricated impedimetric glucose biosensor, with a wide linear range (1-15 mM), high sensitivity (10.6 k omega mM(-1)), and a low detection limit (0.021 mM), was applied successfully for the analysis of real human blood serum samples. The biosensor fabrication was very simple and straightforward.
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