Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume -, Issue -, Pages -Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.2c03139
Keywords
carrageenan; carbohydrate-binding module; fluorescent probe; in situ investigation
Funding
- National Natural Science Foundation of China [31671883]
- Fok Ying-Tong Education Foundation [171024]
- Fundamental Research Funds for the Central Universities [201941005]
- Notes
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In this study, an unknown function module Cgk16A-CBM92 from Cgk16A was cloned and expressed, and it was found to specifically bind to carrageenan. This discovery provides a potential tool for the in situ investigation of carrageenan.
Carrageenan is a commercially important polysaccharide widely applied in the food industry. Specific probes are critical tools for the in situ investigation of polysaccharides, whereas the carrageenan-specific probes are limited at present. Carbohydrate-binding modules (CBMs) could serve as specific probes for the in situ investigation of polysaccharides. In the present study, an unknown function module from the x-carrageenase Cgk16A was cloned and expressed in Escherichia coli. The expressed protein Cgk16A-CBM92 could specifically bind to carrageenan. Its novelty sheds light on a new CBM family (CBM92) as the founding member. Furthermore, a fluorescent probe was successfully constructed by fusing Cgk16A-CBM92 with a green fluorescent protein. The application potential of Cgk16A-CBM92 as a probe served in the in situ visualization of carrageenan was evaluated. The discovery of Cgk16A-CBM92 provided a promising tool for the in situ investigation of carrageenan.
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