Agriterribacter soli sp. nov., isolated from herbicide-contaminated soil

A Gram-stain-negative, non-spore-forming and rod-shaped bacterium, designated strain NS-102(T), was isolated from herbicide-contaminated soil sampled in Nanjing, PR China, and its taxonomic status was investigated by a polyphasic approach. Cell growth of strain NS-102(T) occurred at 16-42 degrees C (optimum, 30 degrees C), at pH 5.0-8.0 (optimum, pH 6.0) and in the presence of 0-3.5 % (w/v) NaCl (optimum, without addition of NaCl). The 16S rRNA gene sequence of strain NS-102(T) shows high similarity to that of Agriterribacter humi YJ03(T) (96.9 % similarity), followed by Terrimonas terrae T16R-129(T) (93.8%) and Terrimonas pekingensis QHT (93.6 %). Average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between the draft genomes of strain NS-102(T) and A. humi YJ03(T) were 72.5, 69.4 and 18.6%, respectively. The only respiratory quinone was MK-7, and phosphatidylethanolamine and unidentified lipids were the major polar lipids. The major cellular fatty acids of strain NS-102(T) contained high amounts of iso-C-15:0 (24.6 %), iso-C(17:0)3-OH (24.1 %), iso-C-15:0 G (16.6 %) and summed feature 3 (C-16:1 omega 6c and/or C-16:1 omega 7c) (15.6%). The G+C content of the total DNA was determined to be 40.0mol%. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain NS-102(T) represents a novel species of the genus Agriterribacter, for which the name Agriterribacter soli sp. nov. is proposed. The type strain is NS-102(T) (=CCTCC AB 2017249(T)=KCTC 62322(T)).

Automated summary

A new bacterium strain, named Agriterribacter soli sp. nov., was isolated from herbicide-contaminated soil in Nanjing, PR China. This strain showed growth at a wide range of temperatures, pH values, and NaCl concentrations. Through morphological, physiological, chemotaxonomic, and phylogenetic analyses, it was clearly distinguished from its closest phylogenetic neighbors.