Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 23, Issue 15, Pages -Publisher
MDPI
DOI: 10.3390/ijms23158788
Keywords
ultra-small iron hydroxide adipate; tartrate coated nanoparticles; HPLC-ICP-MS; genotoxicity; cytotoxicity; Caco-2 cells; HepG2 cells; A2780 cells; GM04312 cells; D; melanogaster
Funding
- government of Principado de Asturias (Oviedo, Spain) through the Science, Technology and Innovation Plan (PCTI) - FEDER funds [SV-PA-21-AYUD/2021/51399]
- Spanish Ministry of Science and Innovation (MCIN) [MCI-20-PID2019-104334RB-100]
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This study investigated the cellular uptake, intracellular dissolution, and biological effects of ultra-small iron hydroxide adipate/tartrate coated nanoparticles in various cell lines. The results showed that these nanoparticles had minimal dissolution after uptake, with DNA damage mainly observed in NER deficient cells. The nanoparticles were found to be a safe alternative for anemia management.
A systematic investigation on the cellular uptake, intracellular dissolution, and in vitro biological effects of ultra-small (<10 nm) iron hydroxide adipate/tartrate coated nanoparticles (FeAT-NPs) was carried out in intestinal Caco-2, hepatic HepG2 and ovarian A2780 cells, and the nucleotide excision repair (NER) deficient GM04312 fibroblasts. Quantitative evaluation of the nanoparticles uptake, as well as their transformation within the cell cytosol, was performed by inductively coupled plasma mass spectrometry (ICP-MS), alone or in combination with high performance liquid chromatography (HPLC). The obtained results revealed that FeAT-NPs are effectively taken up in a cell type-dependent manner with a minimum dissolution after 3 h. These results correlated with no effects on cell proliferation and minor effects on cell viability and reactive oxygen species (ROS) production for all the cell lines under study. Moreover, the comet assay results revealed significant DNA damage only in GM04312 cells. In vivo genotoxicity was further studied in larvae from Drosophila melanogaster, using the eye-SMART test. The obtained results showed that FeAT-NPs were genotoxic only with the two highest tested concentrations (2 and 5 mmol center dot L-1 of Fe) in surface treatments. These data altogether show that these nanoparticles represent a safe alternative for anemia management, with high uptake level and controlled iron release.
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