Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 23, Issue 12, Pages -Publisher
MDPI
DOI: 10.3390/ijms23126391
Keywords
mesenchymal stem; stromal cells; serum-free media; cell proliferation; neuronal differentiation; in vitro studies
Funding
- Current Research Funds [2022]
- Ministry of Health, Italy
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Mesenchymal stem/stromal cells (MSCs) are undifferentiated cells known for their immunomodulatory and regenerative properties. The use of fetal bovine serum (FBS) in culture media poses limitations due to ethical and safety concerns. Serum-free cultures supplemented with growth factors or chemicals can enhance the proliferation and neuronal differentiation of MSCs.
Mesenchymal stem/stromal cells (MSCs) are undifferentiated cells with multilinear potential, known for their immunomodulatory and regenerative properties. Although the scientific community is working to improve their application, concerns limit their use to repair tissues following neurological damage. One of these obstacles is represented by the use of culture media supplemented with fetal bovine serum (FBS), which, due to its xenogenic nature and the risk of contamination, has increased scientific, ethical and safety problems. Therefore, the use of serum-free media could improve MSC culture methods, avoiding infectious and immunogenic transmission problems as well as MSC bioprocesses, without the use of animal components. The purpose of our review is to provide an overview of experimental studies that demonstrate that serum-free cultures, along with the supplementation of growth factors or chemicals, can lead to a more defined and controlled environment, enhancing the proliferation and neuronal differentiation of MSCs.
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