Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 23, Issue 14, Pages -Publisher
MDPI
DOI: 10.3390/ijms23147799
Keywords
octadecyltrichlorosilane; microfluidic; cellulose paper; electrochemical biosensor; chemiresistive; calcium ion
Funding
- Science and Technology Innovation 2030-Key Project of China [2021ZD0113801]
- National Key R&D Program of China [2021YFD2000800, 2019YFD0125600]
- Central Public-interest Scientific Institution Basal Research Fund [2021-YWF-ZYSQ-05]
- State Key Laboratory of Animal Nutrition [2004DA125184G2204]
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In this study, a new method for diagnosing hypocalcemia using a superhydrophobic cellulose-paper-based microfluidic field-effect biosensor was proposed. By improving the hydrophobicity of cellulose paper with OTS, and combining it with single-walled carbon nanotubes and DNAzyme for Ca2+ determination, interference from impurities in blood was effectively reduced.
Hypocalcemia is caused by a sharp decline in blood calcium concentration after dairy cow calving, which can lead to various diseases or even death. It is necessary to develop an inexpensive, easy-to-operate, reliable sensor to diagnose hypocalcemia. The cellulose-paper-based microfluidic field-effect biosensor is promising for point-of-care, but it has poor mechanical strength and a short service life after exposure to an aqueous solution. Octadecyltrichlorosilane (OTS), as a popular organosilane derivative, can improve the hydrophobicity of cellulose paper to overcome the shortage of cellulose paper. In this work, OTS was used to produce the superhydrophobic cellulose paper that enhances the mechanical strength and short service life of MFB, and a microfluidic field-effect biosensor (MFB) with semiconducting single-walled carbon nanotubes (SWNTs) and DNAzyme was then developed for the Ca2+ determination. Pyrene carboxylic acid (PCA) attached to SWNTs through a non-covalent pi-pi stacking interaction provided a carboxyl group that can bond with an amino group of DNAzyme. Two DNAzymes with different sensitivities were designed by changing the sequence length and cleavage site, which were functionalized with SPFET/SWNTs-PCA to form Dual-MFB, decreasing the interference of impurities in cow blood. After optimizing the detecting parameters, Dual-MFB could determine the Ca2+ concentration in the range of 25 mu M to 5 mM, with a detection limit of 10.7 mu M. The proposed Dual-MFB was applied to measure Ca2+ concentration in cow blood, which provided a new method to diagnose hypocalcemia after dairy cow calving.
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