Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 23, Issue 16, Pages -Publisher
MDPI
DOI: 10.3390/ijms23169015
Keywords
Magnaporthe oryzae; MoDHX35; pathogenicity; DExD; H-box protein
Funding
- Zhejiang Provincial Natural Science Foundation [LZ20C140001]
- Zhejiang Key Research and Development Program [2021C02010]
- Bio-health inner-cooperation plan in Zhejiang Academy of Agricultural Sciences
- State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products
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This study reveals the crucial role of DExD/H-box protein MoDHX35 in appressoria formation and virulence of the rice blast fungus, highlighting the involvement of DEAH-box proteins in the pathogenicity of plant fungal pathogens.
The DExD/H-box protein family encompasses a large number of RNA helicases that are involved in RNA metabolism and a variety of physiological functions in different species. However, there is limited knowledge of whether DExD/H-box proteins play a role in the pathogenicity of plant fungal pathogens. In the present work, the DExD/H-box protein MoDHX35, which belongs to the DEAH subfamily, was shown to be crucial in appressoria formation and full virulence of the rice blast fungus, Magnaporthe oryzae. The predicted protein sequence of MoDHX35 had typical DEAH-box domains, showed 47% identity to DHX35 in Homo species, but had no orthologs in Saccharomyces cerevisiae. Deletion of the MoDHX35 gene resulted in reduced tolerance of the mutants to doxorubicin, a nucleic acid synthesis disturbing agent, suggesting the involvement of MoDHX35 in RNA metabolism. MoDHX35-deleted mutants exhibited normal vegetative growth, conidia generation and conidial germination, but showed a reduced appressorium formation rate and attenuated virulence. Our work demonstrates the involvement of DEAH-box protein functions in the pathogenicity of plant fungal pathogens.
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