Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 23, Issue 15, Pages -Publisher
MDPI
DOI: 10.3390/ijms23158630
Keywords
lipolysis-stimulated lipoprotein receptor; cholesterol; astroglia; lipoprotein; apolipoprotein E; siRNA knockdown; qPCR; cell culture
Funding
- Fondation Alzheimer [61]
- Lorraine University of Excellence IMPACT Biomolecules project
- Region Grand Est
- French Ministry of Higher Education, Research and Innovation
- FEDER aid (FRCR 3BR)
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Astroglia plays a critical role in providing cholesterol and ApoE-lipidated particles to neurons, and disruptions in this process can increase the risk of Alzheimer's disease. By modulating the expression of lipolysis-stimulated lipoprotein receptor (LSR) gene, the levels of cholesterol and ApoE output in mouse astrocytes were significantly affected. The findings suggest that LSR acts as a sensor of lipoprotein content in the medium and represses the release of ApoE, while ABCA1 drives cholesterol efflux, potentially impacting cholesterol load, ApoE lipidation, and cholesterol trafficking towards the neuron.
Astroglia play an important role, providing de novo synthesized cholesterol to neurons in the form of ApoE-lipidated particles; disruption of this process can increase the risk of Alzheimer's disease. We recently reported that glia-specific suppression of the lipolysis-stimulated lipoprotein receptor (LSR) gene leads to Alzheimer's disease-like memory deficits. Since LSR is an Apo-E lipoprotein receptor, our objective of this study was to determine the effect of LSR expression modulation on cholesterol and ApoE output in mouse astrocytes expressing human ApoE3. qPCR analysis showed that siRNA-mediated lsr knockdown significantly increased expression of the genes involved in cholesterol synthesis, secretion, and metabolism. Analysis of media and lipoprotein fractions showed increased cholesterol and lipidated ApoE output in HDL-like particles. Further, lsr expression could be upregulated when astrocytes were incubated 5 days in media containing high levels (two-fold) of lipoprotein, or after 8 h treatment with 1 mu M LXR agonist T0901317 in lipoprotein-deficient media. In both conditions of increased lsr expression, the ApoE output was repressed or unchanged despite increased abca1 mRNA levels and cholesterol production. We conclude that LSR acts as a sensor of lipoprotein content in the medium and repressor of ApoE release, while ABCA1 drives cholesterol efflux, thereby potentially affecting cholesterol load, ApoE lipidation, and limiting cholesterol trafficking towards the neuron.
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