Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 23, Issue 16, Pages -Publisher
MDPI
DOI: 10.3390/ijms23169188
Keywords
DNA methylation; epigenetics; autism; organoids; GABA; GAD1; CTCF
Funding
- Psychiatry Research Foundation, Region of Southern Denmark
- Jascha Foundation
- National Institutes of Health BRAIN Initiative [1U01-MH-109091, 5U01-NS-099573]
- Compass Pathways
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DNA methylation analysis has shown promise in identifying biomarkers for neuropsychiatric disorders, and our study found differential methylation patterns in the GAD1 gene of individuals with autism spectrum disorder.
DNA methylation profiling has become a promising approach towards identifying biomarkers of neuropsychiatric disorders including autism spectrum disorder (ASD). Epigenetic markers capture genetic risk factors and diverse exogenous and endogenous factors, including environmental risk factors and complex disease pathologies. We analysed the differential methylation profile of a regulatory region of the GAD1 gene using cerebral organoids generated from induced pluripotent stem cells (iPSCs) from adults with a diagnosis of ASD and from age- and gender-matched healthy individuals. Both groups showed high levels of methylation across the majority of CpG sites within the profiled GAD1 region of interest. The ASD group exhibited a higher number of unique DNA methylation patterns compared to controls and an increased CpG-wise variance. We detected six differentially methylated CpG sites in ASD, three of which reside within a methylation-dependent transcription factor binding site. In ASD, GAD1 is subject to differential methylation patterns that may not only influence its expression, but may also indicate variable epigenetic regulation among cells.
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