Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 23, Issue 12, Pages -Publisher
MDPI
DOI: 10.3390/ijms23126739
Keywords
hypothalamus; periventricular nucleus; dopaminergic neuron; dopamine; L-DOPA; tyrosine hydroxylase; aromatic L-amino acid decarboxylase; third cerebral ventricle; cerebrospinal fluid; rat
Funding
- Russian Science Foundation [20-14-00325]
- IDB RAS Government basic research program [0088-2021-0018]
- Russian Science Foundation [20-14-00325] Funding Source: Russian Science Foundation
Ask authors/readers for more resources
Research has shown that the hypothalamic periventricular nucleus (PeVN) in rats is one of the largest dopamine-rich centers, containing both dopaminergic and monoenzymatic neurons. Evidence was obtained through double immunostaining and high-performance liquid chromatography, demonstrating the presence of thousands of dopaminergic and monoenzymatic neurons in the PeVN.
Since the 1980s, the concept of dopamine-rich brain centers as clusters of only dopaminergic neurons has been fundamentally revised. It has been shown that, in addition to dopaminergic neurons, most of these centers contain neurons expressing one of the enzymes of dopamine synthesis: tyrosine hydroxylase (TH) or aromatic L-amino acid decarboxylase (AADC). We have obtained convincing evidence that in rats, the hypothalamic periventricular nucleus (PeVN) is one of the largest dopamine-rich centers, containing dopaminergic and monoenzymatic neurons. Indeed, using double immunostaining for TH and AADC, the PeVN was shown to contain almost three thousand dopaminergic and monoenzymatic neurons. According to high-performance liquid chromatography, PeVN contains L-DOPA and dopamine, which, apparently, are synthesized in monoenzymatic TH neurons and bienzymatic neurons, respectively. According to confocal microscopy, neurons (cell bodies, fibers), which were immunopositive only to TH, only to AADC, or both, are in close topographic relationships with each other and with the 3rd ventricle. These data suggest the mutual regulation of the neurons, as well as the delivery of dopamine and L-DOPA to the third ventricle, which is confirmed by their detection in the cerebrospinal fluid. Thus, evidence has been obtained that PeVN is one of the largest dopamine-rich centers of the brain, containing dopaminergic and monoenzymatic neurons.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available