Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 23, Issue 15, Pages -Publisher
MDPI
DOI: 10.3390/ijms23158491
Keywords
rice; COMT; lignin; SNP; abiotic stress; expression patterns
Funding
- Heilongjiang Provincial Natural Science Foundation Joint Guidance Project [LH2019C035]
- National Natural Science Foundation of China [U20A2025]
- Northeast Agricultural University Academic Backbone Fund [20XG24]
- Heilongjiang Province Hundred Thousands Major Science and Technology Project of Engineering Biological Breeding [2020ZX16B01]
Ask authors/readers for more resources
In this study, we conducted a systematic study on the rice COMT gene family and found that OsCOMTs play important roles in lignin synthesis and responses to abiotic stress. These findings are significant for future rice breeding and genome editing.
Caffeic acid O-methyltransferase (COMT) is one of the core enzymes involved in lignin synthesis. However, there is no systematic study on the rice COMT gene family. We identified 33 COMT genes containing the methyltransferase-2 domain in the rice genome using bioinformatic methods and divided them into Group I (a and b) and Group II. Motifs, conserved domains, gene structure and SNPs density are related to the classification of OsCOMTs. The tandem phenomenon plays a key role in the expansion of OsCOMTs. The expression levels of fourteen and thirteen OsCOMTs increased or decreased under salt stress and drought stress, respectively. OsCOMTs showed higher expression levels in the stem. The lignin content of rice was measured in five stages; combined with the expression analysis of OsCOMTs and multiple sequence alignment, we found that OsCOMT8, OsCOMT9 and OsCOMT15 play a key role in the synthesis of lignin. Targeted miRNAs and gene ontology annotation revealed that OsCOMTs were involved in abiotic stress responses. Our study contributes to the analysis of the biological function of OsCOMTs, which may provide information for future rice breeding and editing of the rice genome.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available