Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 23, Issue 13, Pages -Publisher
MDPI
DOI: 10.3390/ijms23137463
Keywords
African swine fever virus (ASFV); detection; antibody; indirect ELISA test; surface plasmon resonance (SPR)
Funding
- EU-2020 program [771649]
Ask authors/readers for more resources
African swine fever (ASF) is a dangerous infectious disease that affects domestic and wild pigs, and there are currently no vaccines or effective treatments available. We have developed a specific immunological SPR-based assay that can directly detect ASFV antigen in liquid samples.
African swine fever (ASF) is one of the most dangerous hemorrhagic infectious diseases that affect domestic and wild pigs. Currently, neither a vaccine nor effective treatments are available for this disease. As regards the degree of virulence, ASFV strains can be divided into high, moderate, or low virulence. The main detection methods are based on the use of the polymerase chain reaction (PCR). In order to prevent an uncontrolled spread of ASF, new on-site techniques that can enable the identification of an early-stage disease are needed. We have developed a specific immunological SPR-based assay for ASFV antigen detection directly in liquid samples. The developed assay allows us to detect the presence of ASFV at the dose of 10(3) HAD(50)/(mL).
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available