4.7 Article

A Quantitative Assay for Ca2+ Uptake through Normal and Pathological Hemichannels

Journal

Publisher

MDPI
DOI: 10.3390/ijms23137337

Keywords

connexins; genetically encoded calcium indicators; monoclonal antibodies; drug discovery; genodermatoses; cancer; lentivirus; bicistronic vectors

Funding

  1. University of Padova [BIRD187130]
  2. Fondazione Telethon [GGP19148]
  3. Italian Ministry of Education, Universities and Research (MUR) [PRIN 20175C22WM, PRIN 2017FTJ5ZE]

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This study presents an all-optical assay for measuring ionized calcium uptake, which allows for the tracking of changes in cytosolic calcium concentration. The assay was demonstrated on cells expressing different Cx proteins and showed potential applications in evaluating HC inhibitors and studying HC expression in cells and tissues.
Connexin (Cx) hemichannels (HCs) are large pore hexameric structures that allow the exchange of ions, metabolites and a variety of other molecules between the cell cytoplasm and extracellular milieu. HC inhibitors are attracting growing interest as drug candidates because deregulated fluxes through HCs have been implicated in a plethora of genetic conditions and other diseases. HC activity has been mainly investigated by electrophysiological methods and/or using HC-permeable dye uptake measurements. Here, we present an all-optical assay based on fluorometric measurements of ionized calcium (Ca2+) uptake with a Ca2+-selective genetically encoded indicator (GCaMP6s) that permits the optical tracking of cytosolic Ca2+ concentration ([Ca2+](cyt)) changes with high sensitivity. We exemplify use of the assay in stable pools of HaCaT cells overexpressing human Cx26, Cx46, or the pathological mutant Cx26G45E, under control of a tetracycline (Tet) responsive element (TRE) promoter (Tet-on). We demonstrate the usefulness of the assay for the characterization of new monoclonal antibodies (mAbs) targeting the extracellular domain of the HCs. Although we developed the assay on a spinning disk confocal fluorescence microscope, the same methodology can be extended seamlessly to high-throughput high-content platforms to screen other kinds of inhibitors and/or to probe HCs expressed in primary cells and microtissues.

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