Effect of nickel concentration on biohydrogen production: Organic solid waste vs. glucose

In recent years, alternative renewable energy generation sources have been investigated, highlighting the dark fermentation process due to it's potential to obtain hydrogen-rich gas, which can be used as an energy source. Different trace metals intervene in this biological process. Nickel is one of the most important because it is a component of the [Ni-Fe] hydrogenase enzyme that catalyzes the oxidation of H-2 in numerous bacteria. The aim of this study was to evaluate the effect of nickel on biohydrogen production from organic solid waste (OSW). The experimental setup was carried out in batch tests using OSW as the substrate, glucose as a reference compound and the valuation of Ni2+ doses on the operation in a Sequencing Batch Reactor. The results of the batch tests showed that when using glucose as a substrate, 2 mg Ni2+/g VSinoculum generated the highest hydrogen production (774 +/- 7.3 mL H-2/L/d) and highest yield (55.8 +/- 3.4 mL H-2/g of glucose), which was 34.4% higher than the control. Testing of different concentrations of nickel using OSW as a carbon source showed that the highest production was obtained without Ni2+ addition since the nickel concentration in the residue was 0.17 +/- 0.06 mgNi/gVS; consequently, hydrogen production was not affected by the lack of Ni. The addition of 0.5 mg Ni2+/g VSinoculum decreased acetate and butyrate production and increased caproate production. (C) 2022 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.

Automated summary

In recent years, alternative renewable energy generation sources, particularly the dark fermentation process, have been investigated for their potential in obtaining hydrogen-rich gas. Nickel, as a component of the [Ni-Fe] hydrogenase enzyme, plays a crucial role in catalyzing the oxidation of H-2 in bacteria. This study evaluates the effect of nickel on biohydrogen production from organic solid waste (OSW) and highlights its impact on bacterial metabolism and hydrogen production efficiency.