Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 214, Issue -, Pages 140-151Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2022.05.200
Keywords
Procambarus clarkii; Dopa decarboxylase; Dopamine receptor; RNAi; Neuroendocrine immune system
Funding
- National Natural Science Foundation of China [32060834]
- Natural Science Foundation of Inner Mongolia Autonomous Region of China [2020JQ03]
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The expression patterns of Pc-dar were investigated after bacterial challenge, and it was found to be related to antibacterial innate immunity. Knocking down Pc-ddc resulted in upregulation of Pc-dar expression and inhibited upregulation of endogenous Pc-DDC enzymatic activities after bacterial stimulation. Melanization in crayfish hemolymph was downregulated after bacterial challenge, and Toll signaling pathway and ROS-related genes were activated in response to bacterial stimulation when Pc-ddc was knocked down.
Dopa is decarboxylated by dopa decarboxylase (DDC) to form dopamine, which is a significant signaling molecule in the neuroendocrine system. The dopamine receptor (DAR) is an important transmembrane receptor responsible for receiving extracellular signals in the DAR-mediated signaling pathway. In the present study, the expression patterns of Pc-dar were investigated after bacterial challenge. The obviously changed expression patterns showed Pc-dar was related to the antibacterial innate immunity. Endogenous Pc-DDC enzymatic activities were obviously downregulated after Pc-ddc dsRNA injection. The expression level of Pc-dar mRNA was obviously upregulated after bacterial injection when the expression level of Pc-ddc was knocked down. In addition, the upregulation trend of endogenous Pc-DDC enzymatic activities was obviously restrained after bacterial stimulation when Pc-ddc was knocked down. Finally, melanization was downregulated in crayfish hemolymph compared with the dsGFP injection group. In the RNAi assay, the results of qRT-PCR showed that Toll (TLRs) signaling pathway-related genes were activated in the early stages of bacterial stimulation when Pcddc was knocked down. Four tested ROS-related antioxidant enzyme genes were significantly upregulated after bacterial challenge compared with the dsGFP injection group. The above results indicated that Pc-DDC and PcDAR play important mediating roles in the neuroendocrine immune (NEI) system of crayfish.
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