Biosynthesis of Ag and TiO2 nanoparticles and the evaluation of their antibacterial activities

In this study, Pseudomonas aeruginosa was used for the synthesis of silver and titanium oxide nanoparticles (Ag and TiO2 2NPs). The nanoparticles were prepared by mixing the supernatant solution of the cultured organism and the solution of silver nitrate and titanium tetrabutoxide. UV-Vis spectroscopic analysis gave absorption peak at wavelength of 414 nm and 390 nm for Ag and TiO2 NPs respectively. The FTIR spectra gave absorption bands attributed to N-H stretch of the peptide linkages, O-H stretch of carboxylic acid, amide bend, and -C=O stretch which may have emanated from the micro-organism. The powdered X-ray diffraction (PXRD) studies revealed the crystalline nature of AgNPs and TiO2 NPs while microscopic studies showed spherical and dispersed morphologies with average particle size of 7.27 nm and 6.83 nm for Ag and TiO2 NPs respectively. Antibacterial analysis of the synthesized nanoparticles showed broad spectrum inhibition against disease causing microorganisms with TiO(2)NPs exhibiting excellent results more than AgNPs and the control drugs.

Automated summary

Pseudomonas aeruginosa was used for the synthesis of silver and titanium oxide nanoparticles, and the nanoparticles were characterized by various analytical techniques. The study revealed that TiO2 NPs exhibited superior antibacterial properties.