Journal
IMMUNOLOGY LETTERS
Volume 248, Issue -, Pages 37-44Publisher
ELSEVIER
DOI: 10.1016/j.imlet.2022.06.006
Keywords
Mast cells; MRGPRB2; Calcium ions; ORAI; CACCS
Categories
Funding
- National Natural Science Foundation of China [31771163]
- keyproject of science and technology development plan of traditional Chinese medicine in Jiangsu Province [KYCX21_1745]
- Innovative Project of postgraduate edu-cation in Jiangsu Province
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The activation of MRGPRB2 leads to an increase in intracellular calcium concentration and voltage-dependent current, which is correlated with extracellular calcium concentration. The activation of MRGPRB2 in mast cells involves the PLC-IP3-ORAI signaling pathway and CACCS.
A novel mast cell-specific G-protein-coupled receptor (GPCR), known as Mas-related G protein-coupled receptor-B2 (MRGPRB2), plays important roles in immune response. However, the opening of ion channels mediated by MRGPRB2 activation remains unclear. In this study, we found that [Ca2+]i elevation and voltage-dependent current generated by MRGPRB2 activation were correlated with extracellular calcium concentration. The increases in [Ca2+]i and voltage-dependent current caused by MRGPRB2 activation were blocked by U73122 (PLC blocker) or 2-APB (IP3 blocker) or synta66 (ORAI blocker). The voltage-dependent current induced by MRGPRB2 was inhibited by calcium-activated chlorine channel (CACCS) blockers, DIDS, or NPPB. Our results indicated the involvement of the PLC-IP3-ORAI signaling pathway and CACCS in MRGPRB2-mediated mast cell activation.
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