Journal
GENE
Volume 830, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.gene.2022.146521
Keywords
Brucella abortus vaccine strain A19; Brucella abortus virulent strain 9-941; Comparative genomic analysis; Virulence attenuation
Categories
Funding
- Project of Inner Mongolia Higher Education Researc Agency [NJZZ19041]
- Project of Inner Mongolia Science and Technology Agency [201502071]
- Agricultural Project of Inner Mongolia Science and Technology Agency [20120244]
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This study compared the genome-wide sequence of the live attenuated Brucella abortus A19 strain with a virulent reference strain 9-941, aiming to identify candidate virulence genes and understand the mechanisms behind virulence attenuation. The analysis revealed deletion regions in the A19 genome and minor mutations in important virulence-related genes. Additionally, a PCR differential diagnosis method was developed to distinguish between different Brucella strains. These findings provide valuable resources for further research on other Brucella vaccines and contribute to distinguishing between vaccine immunity and virulent strain infection.
Background: Brucellosis is a widespread disease that affects animals and humans. The live attenuated Brucella abortus A19 strain is used for vaccination against brucellosis in China. In addition, the main mechanisms supporting the residual toxicity of A19 have not been elucidated. Here, we performed a comprehensive comparative analysis of the genome-wide sequence of A19 against the whole genome sequences of the published virulent reference strain 9-941. The primary objective of this study was to identify candidate virulence genes by systematically comparing the genomic sequences between the two genomes. Results: This analysis revealed two deletion regions in the A19 genome, in which all included large fragments of 63 bp, and one of their gene function is related to ABC transporter permease protein. In addition, we have identified minor mutations in important virulence-related genes that can be used to determine the underlying mechanisms of virulence attenuation. The function of its virulence gene covers LysR family transcriptional regulator, outer membrane, MFS transporter and oxidoreductase etc. At the same time, a PCR differential diagnosis method was constructed, which can distinguish A19, S19 and most other commonly used Brucella viruent strains and vaccine strains. Conclusion: The data may help to provide resources for further detailed analysis of mechanisms for other Brucella vaccines. It laid the foundation for further distinguishing between vaccine immunity and virulent strains infection.
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