Evaluation of genetic diversity of colistin-resistant Acinetobacter baumannii by BOX-PCR and ERIC-PCR: the first report

Aim: To control the spread of Acinetobacter baumannii in hospitals, it is necessary to identify the reservoir of organisms and the way they are transmitted. This study analyzed samples by BOX-PCR and enterobacterial repetitive intergenic consensus PCR techniques. Methods: Isolated strains were identified using the Microgen kit and blaOXA-51 gene. The genetic diversity of strains that were sensitive or resistant to colistin was evaluated by BOX-PCR and enterobacterial repetitive intergenic consensus PCR methods. Results: A total of 13% of the isolates were resistant to colistin, whereas 87% of the strains were sensitive to this medication. A. baumannii strains that were resistant or sensitive to colistin were divided into five groups using the BOX-PCR method and six groups using the enterobacterial repetitive intergenic consensus PCR method. Conclusion: Rapid identification and the use of appropriate tools to control colistin-resistant clones are essential to prevent the further spread of A. baumannii.

Automated summary

In order to control the spread of Acinetobacter baumannii in hospitals, it is crucial to determine the source and transmission of the bacteria. This study used BOX-PCR and enterobacterial repetitive intergenic consensus PCR techniques to analyze samples. The results showed that 13% of the isolates were resistant to colistin, while 87% were sensitive. The strains were divided into different groups using the two PCR methods. Rapid identification and the use of appropriate tools are essential in preventing the further spread of colistin-resistant A. baumannii.