4.7 Article

Differentiation of three commercial tuna species through Q-Exactive Orbitrap mass spectrometry based lipidomics and chemometrics

Journal

FOOD RESEARCH INTERNATIONAL
Volume 158, Issue -, Pages -

Publisher

ELSEVIER
DOI: 10.1016/j.foodres.2022.111509

Keywords

Tuna; Lipidomic; Mass spectrometry; OPLS-DA; Biomarker

Funding

  1. Foundation of Hainan Provincial Natural Science Foundation of China [321CXTD1012]
  2. Scientific Research Foundation of Hainan Tropical Ocean University [RHDRC202117]
  3. Ningbo science and technology project [NSFC31871868, 202002N3071]
  4. Scientific Research Program of the General Administration of Customs P.R. China [2020HK209]
  5. Qingdao Customs District Scientific Research Program [QK202101]

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This study analyzed the lipid profiles of three commercial tuna species using UPLC-Q-Exactive Orbitrap MS. It identified 27 potential lipid biomarkers that can effectively differentiate different tuna species.
Mislabeling and adulteration of tuna are common due to the diminishing of morphological characteristics during processing. The tuna authenticity is now being focused in the seafood supply chain. In this study, the lipid profiles of 3 commercial tuna species (skipjack tuna, bigeye tuna and yellowfin tuna) were investigated via ultrahigh performance liquid chromatography coupled with Q-Exactive Orbitrap mass spectrometry (UPLC-Q-Exactive Orbitrap MS). A total of 439 lipid species were identified and semi-quantitated by MS-DIAL. Further biomarkers discovery was carried out by chemometrics, leading to 27 lipids being identified as potential lipid biomarkers. Comparisons to reference standards revealed that lipid biomarkers were effective for discrimination of different tuna species. Interestingly, the proposed lipid biomarkers were all glycerophospholipids, implying that they might be the focus of future study.

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