Journal
FOOD CHEMISTRY
Volume 383, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2022.132598
Keywords
Aflatoxin M-1; Anti-idiotypic nanobody; Chronoamperometry; Milk; Screen-printed carbon electrodes
Funding
- National Key R&D Program of China [2019YFC1606600]
- National Natural Science Foundation of China [32102089]
- Agricultural Science and Technology Innovation Program of CAAS [CAAS-XTCX2019024]
- Funds for basic scientific research of public welfare research institutes [1610172019011]
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This study successfully devised a nontoxic electrochemical immunosensor for the quantitative determination of aflatoxin M-1. The immunosensor demonstrated good selectivity and sensitivity, making it suitable for practical applications such as milk samples.
This study aimed to devise a nontoxic electrochemical immunosensor to quantitatively determine aflatoxin M-1 by chronoamperometry with novel anti-idiotypic nanobody-functionalized screen-printed carbon electrodes (SPCEs). Anti-idiotype nanobodies (AIdnb) were developed to replace the high toxic chemically synthesized antigen. AIdnb was immobilized on the surface of SPCE via covalent coupling as capture reagent. The functionalized SPCEs were followed by characterization using electrochemical impedance spectroscopy, fourier-transform infrared spectroscopy, transmission electron microscopy mapping, and atomic force microscopy. After optimizing experimental parameters, the assembled immunosensor exhibited a good linearity range of 0.25-5.0 ng/mL, with the limit of detection of 0.09 ng/mL. The immunosensor showed a satisfactory selectivity to AFM1, without interference from analogs, including zearalenone, ochratoxin, and fumonisin B-1. For practical application, the developed immunosensor was validated using real spiked samples with the recovery range 82.0%-108.0% and relative standard deviation (RSD) 10.1%-13.0%, indicating that it could be used in milk samples.
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