Journal
FOOD CHEMISTRY
Volume 383, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2022.132456
Keywords
Isomaltooligosaccharides; Dietary fiber; Mammalian alpha-glucosidases; Slowly digestible carbohydrates; Glucose generation rate
Funding
- Nestle Research Project - Nestle Research at Lausanne, Switzerland [DUND-102155]
- Gachon University research fund [GCU-202008490006]
- National Science and Engineering Research Council of Canada (NSERC) through a Discovery Grant
Ask authors/readers for more resources
This study found that mammalian alpha-glucosidases fully hydrolyzed Isomaltooligosaccharides (IMOs) to glucose, although at a slow rate, while AMG could not fully hydrolyze IMOs due to its very low hydrolytic activity on alpha-1,6 linkages.
Isomaltooligosaccharides (IMOs) have been characterized as dietary fibers that resist digestion in the small intestine; however, previous studies suggested that various alpha-glycosidic linkages in IMOs were hydrolyzed by mammalian alpha-glucosidases. This study investigated the hydrolysis of IMOs by small intestinal alpha-glucosidases from rat and human recombinant sucrase-isomaltase complex compared to commonly used fungal amyloglucosidase (AMG) in vitro. Interestingly, mammalian alpha-glucosidases fully hydrolyzed various IMOs to glucose at a slow rate compared with linear maltooligosaccharides, whereas AMG could not fully hydrolyze IMOs because of its very low hydrolytic activity on alpha-1,6 linkages. This suggests that IMOs have been misjudged as prebiotic ingredients that bypass the small intestine due to the nature of the assay used. Instead, IMOs can be applied in the food industry as slowly digestible materials to regulate the glycemic response and energy delivery in the mammalian digestive system, rather than as dietary fibers.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available