Journal
FOOD AND AGRICULTURAL IMMUNOLOGY
Volume 33, Issue 1, Pages 498-510Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540105.2022.2095986
Keywords
Cypress tree bark; B16F10 cells; melanogenesis; CREB/MITF: tyrosinase
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Cypress tree bark extract has demonstrated potential effects against melanogenesis by suppressing the expression of related proteins and mRNA, as well as regulating the activation of CREB.
Cypress tree (Chamaecyparis obtusa) bark is well-known for its bio-functional activities and high content of polyphenol and flavonoids. It has previously exhibited antioxidant, anti-pathogenic, and anti-inflammatory activities. Our study aimed to investigate the anti-melanogenic effect of Cypress Tree Bark extract (CBE). We evaluated cellular tyrosinase activity and melanin content in alpha-melanocyte-stimulating hormone (alpha-MSH) induced B16F10 murine melanoma cells. We analyzed microphthalmia-associated transcription factor (MITF), tyrosinase-related protein (TRP1 and TRP2), and cAMP response element-binding protein (CREB) activation via phosphorylation of AKT and ERK using western blot analysis. Tyrosinase, MITF, TRP1, and TRP2 mRNA expression were examined via real-time polymerase chain reaction. CBE restored melanin content and tyrosinase activity remarkably in alpha-MSH stimulated melanoma cells. It exhibited an anti-melanogenic effect through suppressing MITF, TRP1, TRP2, tyrosinase mRNA and protein expression in alpha-MSH-induced B16F10 cells. Furthermore, CBE has significantly inhibited CREB activation by suppressing AKT and extracellular signal-regulated kinase phosphorylation. Our data strongly suggest that CBE has potential effects against melanogenesis. [GRAPHICS]
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