Journal
FEBS JOURNAL
Volume 290, Issue 1, Pages 196-208Publisher
WILEY
DOI: 10.1111/febs.16592
Keywords
CLR; C-mannosylation; migration; RAMP1; stability
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C-mannosylation is a unique type of protein glycosylation that affects protein functions, including secretion, intracellular localization, and stability. This study identifies a new C-mannosylated protein, RAMP1, and demonstrates that C-mannosylation enhances protein stability and cell migration activity.
C-mannosylation is a unique type of protein glycosylation via C-C linkage between an alpha-mannose and a tryptophan residue. This modification has been identified in about 30 proteins and regulates several functions, such as protein secretion and intracellular localization, as well as protein stability. About half of C-mannosylated proteins are categorized as proteins containing thrombospondin type 1 repeat domain or type I cytokine receptors. To evaluate whether C-mannosylation broadly affects protein functions regardless of protein domain or family, we have sought to identify other types of C-mannosylated protein and analyse their functions. In this study, we focused on receptor activity modifying protein 1, which neither contains thrombospondin type 1 repeat domain nor belongs to the type I cytokine receptors. Our mass spectrometry analysis demonstrated that RAMP1 is C-mannosylated at Trp(56). It has been shown that RAMP1 transports to the plasma membrane after dimerization with calcitonin receptor-like receptor and is important for ligand-dependent downstream signalling activation. Our results showed that C-mannosylation has no effect on this transport activity. On the other hand, C-mannosylation did enhance protein stability and cell migration activity. Our data may provide new insight into both C-mannosylation research and novel RAMP1 analysis.
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