4.1 Article

Metabolic profile of exhaled breath condensate from the pneumonia patients

Journal

EXPERIMENTAL LUNG RESEARCH
Volume 48, Issue 4-6, Pages 149-157

Publisher

TAYLOR & FRANCIS INC
DOI: 10.1080/01902148.2022.2078019

Keywords

3-Sulfinoalanine; exhaled breath condensate; metabonomic; pneumonia

Funding

  1. Beijing Natural Science Foundation [L202007]
  2. Haidian Original Innovation [L202007]
  3. National Major Science and Technology Projects [2018ZX10732401-003]
  4. Beijing Haidian District Preventive Medicine Fund [2017HDPMA04]
  5. Peking University Medicine Fund of Fostering Young Scholars' Scientific & Technological Innovation [BMU2021PY005]
  6. Fundamental Research Funds for the Central Universities
  7. Peking University Health Science Center [BMU2021YJ041]

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This study investigated the application of exhaled breath condensate (EBC) in pneumonia diagnosis using metabolomics analysis. Through LC-MS/MS analysis, 25 differential metabolites were identified, with taurine and hypotaurine metabolism pathway showing the highest correlation with pneumonia development.
Purpose of the Study Exhaled breath condensate (EBC) is increasingly being used for disease diagnosis and environmental exposure assessment as a noninvasive method reducing the risk of exposure. The purpose of this study was to investigate the application of a new sample type of EBC in pneumonia by metabolomics and to explore differential metabolites and potential metabolic pathways. Materials and Methods A case-control study was performed at the Peking University Third Hospital from August to December 2020. C-MS/MS analyses were performed on EBC samples using a UHPLC system. Results Totally 22 patients with pneumonia and 24 healthy controls were recruited. Using untargeted metabolomics based on LC-MS/MS analysis, 25 kinds of differential metabolites were found. Through a comprehensive analysis of the pathways in which the differential metabolites were located, the key pathway with the highest correlation with the difference of metabolites was taurine and hypotaurine metabolism. Conclusions The study implicates that the hypotaurine/taurine metabolic pathway may play a role on the development of pneumonia through metabolism analysis on EBC and the 3-Sulfinoalanine may be used as a biomarker in the diagnosis of pneumonia.

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